Become qualified to perform and teach DOT specimen collections with AlcoPro’s Online DOT Urine and Oral Fluid Specimen Collector Instructor Training. This interactive course covers current 49 CFR Part 40 requirements, collector procedures, chain of custody, collection site security, problem collections, and the proficiency standards required for DOT drug testing professionals.
Choose urine specimen collector instructor training, oral fluid specimen collector instructor training, or dual training in both collection methods. After completing the online regulations portion, you will join a live online mock collections session to complete the required five error-free mock collections and receive practical instruction on how to train and evaluate future collectors.
This course is designed for professionals who need DOT specimen collector certification and instructor certification in one program. Students receive collector and collector instructor certificates, downloadable training materials, instructor guidance, and resources to help train others in compliance with DOT procedures. Oral fluid students also receive training to proficiency on the applicable oral fluid collection device.
Best for third-party administrators, collection site personnel, mobile collectors, occupational health staff, consortiums, and employers who want in-house DOT specimen collector training capability.
DOT-Compliant Specimen Collector Instructor Certification Online
This online DOT specimen collector instructor training course prepares you to perform DOT urine and oral fluid specimen collections and to train other collectors in accordance with 49 CFR Part 40. The program combines regulations training, collection procedures, instructor preparation, and a live proficiency component so you can complete qualification and instructor development in one place.
What’s Included in this DOT Instructor Training Course
Your enrollment includes self-paced online training, live online mock collections, instruction on collector responsibilities, chain of custody form completion, collection site requirements, problem collection scenarios, and instructor materials to help you train future students. Students may select urine, oral fluid, or combined urine and oral fluid instructor training.
Included Certifications
- DOT Specimen Collector Certificate
- DOT Specimen Collector Instructor Certificate
Included Training Components
- 49 CFR Part 40 regulations training
- Five live mock collections
- Instructor manual and teaching resources
- Training slides and procedural support documents
Urine Specimen Collector Instructor Training Topics
Urine training covers DOT-regulated agencies, collector qualifications, collection site setup, security procedures, Federal Custody and Control Form completion, uneventful collections, monitored and direct observation collections, and problem urine collection scenarios.
Oral Fluid Specimen Collector Instructor Training Topics
Oral fluid training covers DOT oral fluid collection procedures, oral cavity inspection, chain of custody requirements, problem collections such as dry mouth and discoloration, documentation when switching testing methods, and proficiency training on the Quantisal II oral fluid collection device.
How DOT Qualification Works
DOT collector qualification requires formal training plus a proficiency demonstration. After you complete the online course content, you will register for a live online mock collections session and complete the required five consecutive error-free mock collections. This process supports qualification for DOT specimen collection work and helps prepare you to train others effectively.
Who This Online DOT Instructor Course Is For
This course is ideal for collectors, collection site managers, TPAs, occupational health professionals, mobile testing providers, employers building internal training capability, and organizations that need a qualified DOT specimen collector instructor for urine, oral fluid, or both.
Why Choose AlcoPro for DOT Specimen Collector Instructor Training
AlcoPro’s course combines interactive online learning, live mock collections, instructor-focused resources, and a Training Success Guarantee. Students receive practical guidance, downloadable tools, and training materials designed to support real-world collection work and future instruction.
Technical Requirements to take this course:
- A computer with speakers and a web camera (built-in or external)
- An internet connection
- This course is best viewed using Google Chrome browser (do not use IE, Edge or Safari browsers to view this course)
- Adobe PDF reader capabilities (recommended to view resource documents)
Supplies Required for Mock Collections
- Urine Collector Instructor Required Supplies
- 8, Federal Custody and Control Forms (use your own or purchase our kits)
- 6, DOT approved split specimen collection kits (use your own or purchase our kits)
- Warm water (to serve as the specimen)
- A donor or subject (the DOT requires you perform mock tests on a live subject)
- Mock session and instructor resources (downloaded from your online course page)
- Disposable gloves (recommended)
- Oral Fluid Collector Instructor Required Supplies
- 8, Federal Custody and Control Forms (use your own or purchase our kits)
- 8, Quantasal II oral fluid testing kits (use your own or purchase our kits)
- A donor or subject (the DOT requires you perform mock tests on a live subject)
- Mock session and instructor resources (downloaded from your online course page)
- Disposable gloves (required)
Frequently Asked Questions
What is included in the Online DOT Specimen Collector Instructor Training course?
This course includes online 49 CFR Part 40 regulations training, live mock collections, specimen collector instruction for urine, oral fluid, or both, and instructor resources such as training materials and guidance for teaching future collectors.
Does this course include the 5 required mock collections?
Yes. After you complete the online regulations portion, you will register for a live online mock collections session and complete the five mock collections required for DOT collector qualification.
Can I choose urine training, oral fluid training, or both?
Yes. Students can enroll in urine specimen collector instructor training, oral fluid specimen collector instructor training, or dual training for both specimen collection methods.
Will I receive both collector and instructor certification?
Yes. Students who successfully complete the course requirements receive a DOT Specimen Collector certificate and a Specimen Collector Instructor certificate.
Is this course based on current DOT regulations?
Yes. The training applies current DOT regulations under 49 CFR Part 40 and covers procedural and proficiency requirements for DOT drug testing professionals.
Do oral fluid students receive device-specific proficiency training?
Yes. Oral fluid students must demonstrate proficiency on the applicable oral fluid collection device as part of the training and qualification process.
How long do I have to complete the course?
The course is designed to be taken at your own pace, but students are encouraged to complete the process promptly. Mock collections should be completed within 30 days of finishing the regulations training.
Do I need to retake DOT specimen collector training?
Yes. DOT specimen collector training must be taken every 5 years as refresher training. While there is no DOT requirement to retake instructor training every 5 years, many instructors do so to receive updated instructor training resources and materials.
Question
Mary-Ann Nuesse –
Once I am trained as a DOT Collector Trainer, do I need students to log into any coursework with your company in order for me to certify them, or do I receive materials that I can use to train others as DOT Collectors?
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Jennie Lee-Pace –
Hello Mary-Ann.
Aside from giving you useful tools and tips on teaching the federal regulations, we provide you with an instructor manual with printed training resources, as well as PowerPoint slides to to train and certify your students. Some of our instructors choose to put their trainees through our online regulations course and spend their time overseeing the mock collections. We extend our online regulations course to our instructors at a fraction of the cost.
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Ashley Duran / Dr. M. Nuesse –
Thank you for responding to our questions. What is the cost for the courses?
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Jennie Lee-Pace –
The online training course is $498 and our classroom training is $525. Please let me know how I may be of continued service.
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Question
Angel Williams –
How long do I have to complete this course?
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Jennie Lee-Pace –
Hello Angel.
This course is designed to learn at your pace. However, we do encourage you to try to get everything done within 30-days. We find student’s who stretch it out over too much time, start to loose the information they learn. The only DOT requirement is that you conduct your mock collections within 30-days of completing the regulations training online. The mock collections are done during a live training event using WebEx. You register for the live training once you complete regulations. I hope this is helpful information.
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Quinton Munro –
As the title says, really good course with very nice, experienced, knowledgable people running it. Fabienne helped me with sign up and any questions I had about ordering supplies, it seems like I always get a response with in an hour of me emailing her. Ken is the one who runs the course, the powerpoint slides are great, they are very easy to follow and the information I needed to learn was laid out very well. I did my mock collections with Ken over a video call, I was nervous about it but then when I saw the kind of person Ken is I realized there is no reason to be nervous. He does not want to make things difficult and just wants to see you succeed and make sure that you know all of the information you need to know. My certificate was received immediately after completion of the course via email and a hard copy was received in about two days. I highly recommend this course.
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Jennie Lee-Pace –
Thanks Quinton. Loved having you in our online class.
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Adam Loutsch (verified owner) –
Very easy to navigate and understand. The process was very quick and easy to use.
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Lisa Altman (verified owner) –
From the moment I made my first call to receive information about the DOT Specimen Collector Instructor Training course I was impressed by the quick response and assistance that was offered to me to get certified asap. The online course was detailed as well as easy to follow along. The training videos were helpful and gave a great visual of the step by step procedures that are required by DOT. Fabienne the Mock Training Instructor was fantastic. She guided you through the process step by step and was able to answer all my questions. I would highly recommend Alcopro
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Jennie Lee-Pace –
Hi Lisa.
Thank you for trusting us. So glad you chose us for your training.
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Question
Shalaunda Reese –
Once I complete this class, am I able to conduct Mock Collections for individuals outside of my agency?
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Jennie Lee-Pace –
Yes, with your instructor certificate you are able to train others. We also extend to our instructor students discounts on our online regulations training, so they can put their students through the rules and regulations training online and only have to oversee the 5 mock collections.
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Question
Angela McNeill –
How does becoming a trainer to train employees benefit my organization? Also do I need to purchase 1 mock specimen for all 5 mocks or just 1? Thanks
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Jennie Lee-Pace –
Being trained and certified to train others benefits your organization if they have 3 or more employees in need of training. Our online DOT specimen collector training includes regulations training and the 5 mocks and instructor training and resources. The regular DOT specimen collector with mocks includes regulations and the 5 mock tests. You can speak to a training specialist by calling 800.227.9890.
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Marcy (verified owner) –
I recently completed my DOT specimen collection and Instructor training program and the program was streamlined from start to finish. The staff at Alcopro are wonderful. My instructor was very knowledgeable and made sure I had received all tools needed to be a successful collector and trainer.
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thorntonapn (verified owner) –
The course was in an easy to follow format that was very informative.
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Question
Michelle Lynn Frohn –
once trained is it forever or does it ever expire or any yearly fees
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Jennie Lee-Pace –
In this course you will receive a DOT specimen collector certificate and a collector instructor certificate. The DOT requires you to retake DOT specimen collector training every 5 years. We call this refresher training, which is a repeat of the initial training that you take. There is no DOT requirement to retake instructor training every 5 years. However, many instructors come back to retake the instructor course and receive a renewed specimen collector and instructor certificates. By retaking the instructor course every 5 years, you are provided with updated and current instructor materials.
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