Online DOT Urine & Oral Fluid Specimen Collector Instructor Training

Become qualified to perform and teach DOT specimen collections with AlcoPro’s Online DOT Urine and Oral Fluid Specimen Collector Instructor Training. This interactive course covers current 49 CFR Part 40 requirements, collector procedures, chain of custody, collection site security, problem collections, and the proficiency standards required for DOT drug testing professionals.

Choose urine specimen collector instructor training, oral fluid specimen collector instructor training, or dual training in both collection methods. After completing the online regulations portion, you will join a live online mock collections session to complete the required five error-free mock collections and receive practical instruction on how to train and evaluate future collectors.

This course is designed for professionals who need DOT specimen collector certification and instructor certification in one program. Students receive collector and collector instructor certificates, downloadable training materials, instructor guidance, and resources to help train others in compliance with DOT procedures. Oral fluid students also receive training to proficiency on the applicable oral fluid collection device.

Best for third-party administrators, collection site personnel, mobile collectors, occupational health staff, consortiums, and employers who want in-house DOT specimen collector training capability.

DOT-Compliant Specimen Collector Instructor Certification Online

This online DOT specimen collector instructor training course prepares you to perform DOT urine and oral fluid specimen collections and to train other collectors in accordance with 49 CFR Part 40. The program combines regulations training, collection procedures, instructor preparation, and a live proficiency component so you can complete qualification and instructor development in one place.

What’s Included in this DOT Instructor Training Course

Your enrollment includes self-paced online training, live online mock collections, instruction on collector responsibilities, chain of custody form completion, collection site requirements, problem collection scenarios, and instructor materials to help you train future students. Students may select urine, oral fluid, or combined urine and oral fluid instructor training.

Included Certifications

• DOT Specimen Collector Certificate
• DOT Specimen Collector Instructor Certificate

Included Training Components

• 49 CFR Part 40 regulations training
• Five live mock collections
• Instructor manual and teaching resources
• Training slides and procedural support documents

Urine Specimen Collector Instructor Training Topics

Urine training covers DOT-regulated agencies, collector qualifications, collection site setup, security procedures, Federal Custody and Control Form completion, uneventful collections, monitored and direct observation collections, and problem urine collection scenarios.

Oral Fluid Specimen Collector Instructor Training Topics

Oral fluid training covers DOT oral fluid collection procedures, oral cavity inspection, chain of custody requirements, problem collections such as dry mouth and discoloration, documentation when switching testing methods, and proficiency training on the Quantisal II oral fluid collection device.

How DOT Qualification Works

DOT collector qualification requires formal training plus a proficiency demonstration. After you complete the online course content, you will register for a live online mock collections session and complete the required five consecutive error-free mock collections. This process supports qualification for DOT specimen collection work and helps prepare you to train others effectively.

Who This Online DOT Instructor Course Is For

This course is ideal for collectors, collection site managers, TPAs, occupational health professionals, mobile testing providers, employers building internal training capability, and organizations that need a qualified DOT specimen collector instructor for urine, oral fluid, or both.

Why Choose AlcoPro for DOT Specimen Collector Instructor Training

AlcoPro’s course combines interactive online learning, live mock collections, instructor-focused resources, and a Training Success Guarantee. Students receive practical guidance, downloadable tools, and training materials designed to support real-world collection work and future instruction.

Technical Requirements to take this course:

• A computer with speakers and a web camera (built-in or external)
• An internet connection
• This course is best viewed using Google Chrome browser (do not use IE, Edge or Safari browsers to view this course)
• Adobe PDF reader capabilities (recommended to view resource documents)

Supplies Required for Mock Collections

• Urine Collector Instructor Required Supplies
  • 8, Federal Custody and Control Forms (use your own or purchase our kits)
  • 6, DOT approved split specimen collection kits (use your own or purchase our kits)
  • Warm water (to serve as the specimen)
  • A donor or subject (the DOT requires you perform mock tests on a live subject)
  • Mock session and instructor resources (downloaded from your online course page)
  • Disposable gloves (recommended)
• Oral Fluid Collector Instructor Required Supplies
  • 8, Federal Custody and Control Forms (use your own or purchase our kits)
  • 8, Quantasal II oral fluid testing kits (use your own or purchase our kits)
  • A donor or subject (the DOT requires you perform mock tests on a live subject)
  • Mock session and instructor resources (downloaded from your online course page)
  • Disposable gloves (required)